Carbohydrate Mapping Technology for biomass conversion

There is an urgent need for more and better experimental data in this genomic era. It is generally recognized that biochemists/enzymologists need to speed up the delivery of quality analytical data for high-throughput analysis and enzyme screening to close the gap with the genomic sequences deposited in databases and their bio-informatic annotations. Therefore, platforms e.g. in a 96- or 384-well plate formats are being developed that provide easy, fast, and high-quality quantitation and kinetic data.

With this project, we aim both at the development of high-throughput methods able to perform enzymatic activity assays on representative lignocellulosic biomass samples and the valorisation of the technology.

More specifically, a fast and sensitive assay for the high-throughput screening of GH (glycoside hydrolases) and PMO (polysaccharide mono-oxygenases) enzymes will be presented that is based on a library of biomass-derived oligosaccharides. The final goal is to highlight the strength of our methodology via academic publications and implement the carbohydrate mapping technology in carbohydrate research via a (inter-)national academic and industrial network. In this perspective, the applicant will explore if how the methodology can be marketed (licensed) in the best way.

In conclusion, the developed Carbohydrate Mapping Technology will offer a platform that provides easy, fast, and high-quality quantitation and kinetic data in a 96- or 384-well plate where existing methods have limitations in throughput, performance or cost.

 

Contact: Ingeborg Stals

Funding: Ghent University
Supervisor: Prof. Dr. A Van Landschoot

Co-supervisor: Prof. Dr. T. Desmet

Period: 2013 - 2015