Applied Flow Cytometry – (pre)clinical hematological analyses

Clinical hematology

Target group

Members of the Doctoral School of Life Sciences and Medicine

Level

Basic knowledge in flow cytometry

Organizers

  • Prof Dr Evelyne Meyer, Ghent University
  • Prof Dr Eric Cox, Ghent University
  • Prof Dr Marc De Waele, VUB
  • Dr Nathalie Cools, Antwerp University
  • Prof Dr Viggo Van Tendeloo, Antwerp University

Programme and venu

  • Mon 21 september 2015

Location: Laboratory of Hematology, UZ Brussels, Paviljoen Louis Tielemans, meeting room Klinische Biologie ( level -1), Laarbeeklaan 101 - 1090 Brussel.
Route description: Entrance via the main entrance of UZ Brussel. In the hall, take the corridor at the left (direction cardiovascular diseases). Laboratory for Clinical Biology is seen in front of you. At this lab, go to the right and sign up at the secretary. They will point you the way to the meeting room.
Tel: 02/ 477 50 71

9 - 12 : Flow cytometry in the diagnosis and follow-up of leukemia and lymphoma.
12 - 13: Lunch
13 - 16 : Practical aspects: labeling of the cells, examination with the flow cytometer and interpretation of the data.

  • Tue 22 september 2015

Location: Laboratory of Biochemistry (morning) and Immunology (afternoon), Faculty of Veterinary Medicine, University of Ghent, Salisburylaan 133 - 9820 Merelbeke .
Route description:
Faculty: http://www.ugent.be/di/nl/faculteit/contact/wegbeschrijving.htm
Biochemistry: High Building, Unit A – 3 th floor, meeting room.
Immunology:  High Building, Unit B – 3th floor.
Tel: 09/ 264 73 55 or 0476 57 86 91

Biochemistry:
9:30-10:30: Introduction Multiplex Bead-Based Flow cytometry
10:30-12:00: Explanation on the FacsArray flow cytometer with attention to the pitfalls in FacsArray CBA.

12-13: Lunch

Immunology:
13- 14 : Introduction to fluorescent activated cell sorting (FACS)
14-16u30: Practical aspects: explanation of the sorting process on the instrument by the responsible of the FACSAria III celsorter,  including ‘tips and tricks’ to perform an efficient sort.

  • Wed 23 september 2015

Location: Laboratory of Experimental Hematology, Vaccine  & Infectious Disease Institute (VAXINFECTIO), Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijkstraat 10 - 2650 Edegem.
Route description UZA
Tel: 03/ 821 35 84

10-12: Detection of antigen specific T cells using intracellular cytokine determination (ICS).
12-13: Lunch
13-17: Practical aspects: performing protocol (fix/ perm) + antibody staining, followed by flowcytometric analysis with attention for pitfalls at ICS.

Registration fee

Free of charge for the selected participants

Registration procedure

To register or to add your name to the waiting list, send an e-mail to doctoralschools@UGent.be (mentioning your name, first name, student number, Doctoral School, Department and the course title). Your registration will be confirmed by e-mail.

Language

English

Number of participants

6

Evaluation criteria (doctoral training programme)

Presence + active participation