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Prof. dr. Kevin Braeckmans

Kevin Braeckmans

Contact info

Kevin Braeckmans

Laboratory of General Biochemistry & Physical Pharmacy
Harelbekestraat 72, B-9000 Ghent, Belgium

Phone: +32 9 264 80 98

Fax: +32 9 264 81 89


E-mail: Kevin.Braeckmans@UGent.be

 

Having obtained a Master degree in Physics at Ghent University (Belgium) in 1999, he joined the Laboratory of General Biochemistry and Phsyiscal Pharmacy (Ghent University) to perform research on advanced optical microscopy methods for pharmaceutical applications. During his Ph.D. he was involved in the development of a new type of encoded microcarriers for diagnostic applications. This research contributed to the establishment of a spin-off company Memobead Technologies, which now has been taken over by a Swiss company (Biocartis). For this work he has received the first price for Young Biotechnology Researcher from the Funds of Biotechnology (FBBF, Belgium) in 2005. During his Ph.D. he also performed research on the development of new methods for quantitative fluorescence recovery after photobleaching (FRAP) measurements in biological and pharmaceutical materials. Having obtained his Ph.D. in 2004, he received a fellowship of the Fund for Scientific Research – Flanders as a post-doctoral researcher. In 2005 he joined the group of prof. Braüchle (Lehrstuhl für Physikalische Chemie I) at the Ludwig-Maximilians Universität München where he was involved in the development of algorithms and software for single particle tracking analysis. Later on he returned to the Laboratory of General Biochemistry and Physical Pharmacy (Ghent University) to continue his research on advanced microscopy methods. In 2008 he became a professor at the Faculty of Pharmaceutical Sciences of Ghent University where he is currently leading the Biophotonic Imaging Group, which is hosted by the Laboratory of General Biochemistry and Physical Pharmacy. His current research topics involve the development of novel microscopy techniques for measuring molecular dynamics and their application to the study of extra- and intracellular barriers for drug and gene delivery.

Publications

  1. Vandenbosch D., Braeckmans K., Nelis H., Coenye T. Fungicidal activity of miconazole against Candida spp. biofilms. J. Antimicrob. Chemoth. 65, 964-700 (2010).
  2. Vercauteren D., Vandenbroucke R.E., Jones A.T., Rejman J., Demeester J., De Smedt S.C., Sanders N.N., Braeckmans K. The use of inhibitors to study endocytic pathways of gene carriers: Optimisation and pitfalls. Mol. Ther. 18, 561-569 (2010).
  3. Censi R., Vermonden T., van Steenbergen M.J., Deschout H., Braeckmans K., De Smedt S.C., van Nostrum C.F., di Martino P., Hennink W.E. Photopolymerized thermosensitive hydrogels for tailorable diffusion-controlled protein delivery. J. Control. Release 140, 230-236 (2009).
  4. van de Manakker F., Braeckmans K., el Morabit N., De Smedt S.C., van Nostrum C.F., Hennink W.E. Protein Release Behaviour of Self-Assembled PEG-β-cyclodextrin/PEG-cholesterol hydrogels.  Adv. Funct. Mat. 19, 2992-3001 (2009).
  5. Soenen S.J.H., Eszter I., Zsuzsa M., Vercauteren D., Braeckmans K., De Smedt S.C., De Cuyper M. The role of nanoparticle concentration-dependent induction of cellular stress in the internalization of non-toxic cationic magnetoliposomes. Biomaterials 30, 6803-6813 (2009).
  6. Sanders N.N., Rudolph C., Braeckmans K., De Smedt S.C., Demeester J. Extracellular barriers in respiratory gene therapy. Adv. Drug Deliver. Rev. 61, 115-127 (2009).
  7. Soenen S.J.H.,  Vercauteren D., Braeckmans K., Noppe W., De Smedt S., De Cuyper M. Stable lon- term intracellular labelling with fluorescently-tagged cationic magnetoliposomes. ChemBioChem 10, 257-267 (2009).
  8. Mazza D., Braeckmans K. (with equal contribution), Cella F., Testa I., Vercauteren D., Demeester J., De Smedt S.C., Diaspro A.  A new FRAP/FRAPa method for three-dimentsional diffusion measurements based on multiphoton excitation microscopy. Biophys J. 95, 3457-3469 (2008).
  9. Stubbe B.G., Gevaert S., Derveaux S., Braeckmans K., De Geest B.G.G., Goethals M., Vandekerckhove J., Demeester J., De Smedt S.C. Evaluation of encoded layer-by-layer coated microparticles as protease sensors. Adv. Funct. Mat. 18, 1624-1631 (2008).
  10. Derveaux S., Stubbe B.G., Braeckmans K., Roelant C., Demeester J., De Smedt S.C. Synergism between particle-based multiplexing and mircrofluidics Technologies may bring diagnostics closer to the patient. Anal. Bioanal. Chem. 391, 2453-2467 (2008).
  11. Peneva K., Mihov G., Nolde F., Rocha S., Hotta J.I., Braeckmans K., Hofkens J., Uji-I H., Herrmann A., Müllen K. Water-Soluble Monofunctional Perylene and Terrylene Dyes: Powerful Labels for Single-Enzyme Tracking. Angew. Chem. Int. Edit. 47, 3372-3375 (2008).
  12. Buyens K., Lucas B., Raemdonck K., Braeckmans K., Vercammen J., Hendrix J., Engelborghs Y., De Smedt S.C., Sanders N.N. A fast and sensitive method for measuring the integrity of siRNA-carrier complexes in full human serum. J. Control. Release 126, 67-76 (2008).  
  13. Remaut, K., Sanders, N., De Geest, B.G., Braeckmans, K., Demeester, J. and De Smedt, S.C. Nucleic acid delivery: Where material sciences and bio-sciences meet. Mat. Sci. Eng. R. 58, 117-161 (2007).
  14. Derveaux S., De Geest B.G.G., Roelant C., Braeckmans K., Demeester J., De Smedt S.C. Multifunctional layer-by-layer coating of digitally encoded microparticles. Langmuir 23, 10272-10279 (2007).
  15. Fayazpour F., Lucas B., Huyghebaert N., Braeckmans K., Derveaux S., Stubbe B.G., Remon J.P., Demeester J., Vervaet C. and De Smedt S.C. Digitally encoded drug tablets to combat counterfeiting. Adv. Mater. 19, 3854-+ (2007).
  16. Remaut K., Lucas B., Raemdonck K., Braeckmans K., Demeester J., De Smedt S.C. Can we better understand the intracellular behavior of DNA nanoparticles by fluorescence correlation spectroscopy? J. Control. Release 121, 49-63 (2007).
  17. Remaut, K., Lucas, B., Raemdonck, K., Braeckmans, K., Demeester, J. and De Smedt, S.C. Protection of oligonucleotides against enzymatic degradation by pegylated and nonpegylated branched polyethyleneimine. Biomacromolecules 8, 1333-1340 (2007).
  18. Van Thienen T.G., Horkay F., Braeckmans K., Stubbe B.G., Demeester J., De Smedt S.C. Influence of free chains on the swelling pressure of PEG-HEMA and dex-HEMA Hydrogels. Int. J. Pharm.  337, 31-39 (2007).
  19. Geenen K., Nauwynck H.J., De Regge N., Braeckmans K., Favoreel H.W. Brn-3a suppresses pseudorabies virus-induced cell death in sensory neurons. J. Gen. Virol. 88, 743-747 (2007).
  20. Braeckmans K., Remaut K., Demeester J., De Smedt S.C. Line-FRAP with the confocal laser scanning microscope for diffusion measurements in 3-D samples. Biophys. J. 92, 2172-2183 (2007).
  21. Ruenraroengsak P., Al-Jamal K.T., Hartell N., Braeckmans K., De Smedt S.C., Florence A.T. Cell uptake, cytoplasmic diffusion and nuclear access of a 6.5 nm diameter dendrimer. Int. J. Pharm. 331, 215-219 (2007).
  22. Remaut K., Lucas B., Braeckmans K., Demeester J., De Smedt S.C. Pegylation of liposomes favours the endosomal degradation of the delivered phosphodiester oligonucleotides. J. Control. Release 117, 256-266 (2007).
  23. Braeckmans K., Stubbe B.G., Remaut K., Demeester J., De Smedt S.C. Anomalous photobleaching in fluorescence recovery after photobleaching measurements due to excitation saturation—a case study for fluorescein. J. Biomed. Opt. 11, 044013 (2006).
  24. van Drooge D.J., Braeckmans K. (with equal contribution), Hinrichs W.L.J., Remaut K., De Smedt S.C., Frijlink H.W. Characterization of the mode of incorporation of lipophilic compounds in solid dispersions at the nanoscale using fluorescence resonance energy transfer (FRET). Macromol. Rapid. Comm. 27, 1149-1155 (2006).
  25. Bausinger R., von Gersdorff K., Braeckmans K., Ogris M., Wagner E., Bräuchle C., Zumbusch A. The Transport of Nanosized Gene Carriers Unravelled by Live Cell Imaging. Angew. Chem. Int. Edit. 45, 1568-1572 (2006).
  26. Alvarez-Mancenido F., Braeckmans K., De Smedt S.C., Demeester J., Landin M., Martinez-Pacheco R. Characterization of diffusion of macromolecules in konjac glucomannan solutions and gels by fluorescence recovery after photobleaching technique. Int. J. Pharm. 316, 37-46 (2006).
  27. Hinrichs W.L.J., Mancenido F.A., Sanders N.N., Braeckmans K., De Smedt S.C., Demeester J., Frijlink H.W. The choice of a suitable oligosaccharide to prevent aggregation of PEGylated nanoparticles during freeze thawing and freeze drying. Int. J. Pharm. 311, 237-244 (2006).
  28. Remaut K., Lucas B., Braeckmans K., Sanders N.N., Demeester J., De Smedt S.C. Delivery of phosphodiester oligonucleotides : Can DOTAP/DOPE liposomes do the trick? Biochemistry 45, 1755-1764 (2006).
  29. Peeters L, Sanders N.N., Braeckmans K., De Smedt S.C., Demeester J. Vitreous – a barrier to non-viral ocular gene therapy. Invest. Ophth. Vis. Sci. 46, 3553-3561 (2005).
  30. Remaut K., Lucas B., Braeckmans K., Sanders N.N., Demeester J., De Smedt S.C. Protection of oligonucleotides against nucleases by pegylated and non-pegylated liposomes as studied by fluorescence correlation spectroscopy. J. Control. Release 110, 212-226 (2005).
  31. Van Tomme S.R., De Geest B.G., Braeckmans K., De Smedt S.C., Siepmann F., Siepmann J., van Nostrum C.F., Hennink W.E. Mobility of model proteins in hydrogels composed of oppositely charged dextran microspheres studied by protein release and fluorescence recovery after photobleaching. J. Control. Release 110, 67-78 (2005).
  32. De Geest B.G., Déjunat C., Sukhorukov G.B., Braeckmans K., De Smedt S.C., Demeester J. Self-rupturing microcapsules. Adv. Mater. 17, 2357-2361 (2005).
  33. Lucas B., Remaut K., Sanders N.N., Braeckmans K., De Smedt S.C., Demeester J. Studying the intracellular dissociation of polymer-oligonucleotide complexes by dual color fluorescence fluctuation spectroscopy and confocal imaging. Biochemistry 44, 9905-9912 (2005).
  34. Lucas B., Remaut K., Sanders N.N., Braeckmans K., De Smedt S.C. and Demeester J. Towards a better understanding of the dissociation behavior of liposome-oligonucleotide complexes in the cytosol of cells. J. Control. Release 103, 435-450 (2005).
  35. Remaut K., Lucas B., Braeckmans K., Sanders N.N., De Smedt S.C. and Demeester J. FRET-FCS as a tool to evaluate the stability of oligonucleotide drugs after intracellular delivery. J.  Control. Release 103, 259-271 (2005).
  36. De Smedt S.C., Remaut K., Lucas B., Braeckmans K., Sanders N.N., and Demeester J. Studying biophysical barriers to DNA delivery by advanced light microscopy. Adv. Drug Deliver. Rev. 57, 191-210 (2005).
  37. Lucas B., Remaut K., Braeckmans K., Haustraete J., De Smedt S.C., Demeester J. Studying pegylated DNA complexes by dual color fluorescence fluctuation spectroscopy. Macromolecules 37, 3832-3840 (2004).
  38. Braeckmans K., Peeters L., Sanders N.N., De Smedt S.C., and Demeester J. Three-dimensional fluorescence recovery after photobleaching with the confocal scanning laser microscope. Biophys. J. 85, 2240-2252 (2003).
  39. Braeckmans K., De Smedt S.C., Roelant C., Leblans M., Pauwels R., and Demeester J. Encoding microcarriers by spatial selective photobleaching. Nat. Mater. 2, 169-173
  40. Braeckmans K., De Smedt S.C., Leblans M., Pauwels R., and Demeester J. Encoding microcarriers: Present and future technologies. Nat. Rev. Drug Discov. 1, 447-456 (2002).
  41. Stubbe B.G., Braeckmans K., Horkay F., Hennink W.E., De Smedt S.C., and Demeester J. Swelling pressure observations on degrading dex-HEMA hydrogels. Macromolecules 35, 2501-2505 (2002).