Publication in Nature Communications by Ranhua Xiong


Sizing nanomaterials in complex biological fluids, such as blood, remains a great challenge in spite of its importance for a wide range of biomedical applications. In Nature Communications, Ranhua Xiong et al. reported on continuous Fluorescence Recovery After Photobleaching (cFRAP) as a new analytical method enabling size distribution measurements of nanomaterials (1-100 nm) in undiluted biological fluids. In cooperation with Anabiotec nv (Belgium), we demonstrate that cFRAP allows to measure protein aggregation in human serum. Furthermore,  in cooperation with VIB, we determined the permeability of intestinal and vascular barriers in vivo. cFRAP is a new analytical technique that paves the way towards exciting new applications that benefit from nanomaterial sizing in bio-fluids.
Title: Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability
DOI: 10.1038/ncomms12982