Laboratory of Biochemistry

Research mission

The research mission of the Laboratory of Biochemistry (Prof. dr. E. Meyer) is to contribute to the elucidation of key infectious diseases such as mammary gland infection or mastitis and sepsis and of inflammatory processes such as mammary tumor progression and metastasis, using a validated mouse intraductal injection model.

For these purposes wild type as well as genetically modified (e.g. NF-қB reporter and chitinase 3-like 1 knock-out) mice are used. This preclinical model is also an elegant screening tool for candidate therapeutic molecules being described in more detail in the scientific services section of our group.

In this context (innate) immune cells are analysed and cytokine profiling is typically performed. Complementing this inflammatory profile, Chitinase 3-like 1 - originally patented by our group as a novel sepsis-induced biomarker for acute kidney injury - is evaluated as a novel innate immunity related protein biomarker for infection and inflammation.

The Laboratory of Biochemistry has developed an extensive set of more than 150 standard operation procedures (e.g. SOP’s for 3-laser flowcytometry, cell culture, bacteriology, western blotting, ELISA) to analyze a variety of biological samples such as urine, serum, (mammary gland and other) tissue, synovial fluid and cell culture supernatant obtained from in vivo and in vitro studies.

Doctoral research topics veterinary biochemistry

Research within the veterinary biochemistry group is related to several topics:

  • Involvement of immune cells in breast cancer using an innovative intraductal mouse model, PhD candidate Jonas Steenbrugge
  • Development of a novel therapeutic transport technique in breast cancer using mouse breast cancer models, PhD candidate Francis Combes
  • The role of various coagulase-negative staphylococci for udder health of dairy cows, PhD candidate Chloë Geeroms
  • Chitinase 3-like 1 as a promising parameter in (sepsis-induced) acute kidney injury, PhD candidate Jorien De Loor
  • Investigation of early stages of acute kidney injury caused by Babesia rossi using several urinary biomarkers, PhD candidate Pieter Defauw
  • Use of biocides on pig and poultry farms and their relation to the problem of antimicrobial resistance evaluated by flow cytometric analysis of bacterial isolates, PhD candidate Helder Maertens