Laser Capture Microscope

Difference between two systems of LCM at our departmentLaser capture microdissection enables the isolation of single cells or homogeneous cell clusters for downstream genetic or proteomic analysis. The department of Morphology houses two systems: the PixCeLL II (Arcturus) and a PALM Microbeam IV (ZEISS).

 

 

Laser capture microdissection enables isolation of cells from tisseu sections using a cap with a thermoplastic polymer film. The infrared laser is pointed on the cells of interests and melts the polymer onto these cells. by lifting the cap, the cells of interest that adhere to the melted polymer are isolated and can be used for further analysis.

Detailed view of an histology slide before and after Laser Capture MicrodissectionLaser catapulting enables the isolation of cells by using a UV laser to cut and catapult cells of interest into an overlaying cap. This system has the advantage that no contamination of surrounding tissue is possible.

 

 

PALM Microbeam IV (ZEISS)

The PALM Microbeam uses an ultraviolet laser to cut and catapult cells or tissue fractions from histological sections or from cell cultures.

 

 Image of the PALM ZEISS system

 

Technical details:

Inverted microscope

Robostage for petri dishes and 96 well plate collection

Stage incubator for life cell isolation (no CO2)

 

Lenzes:

Magn/NA: 5x/0.25,10x/0.5, 20x/0.4, 40x/0.6, 63x/0.75, 100x/1.4 (oil);

 

Fluorescence filters:

BFP (Dapi, Hoechst)

FITC

HQ Texas Red

CY5

AopTome.2 optical sectioning by structured illumination (similar axial resolution as confocal)

 

Camera:

Axiocam 506 mono

Axiocam 503 color

 PixCell II (Arcturus)

The PixCell II system uses an infrared laser in combination with thermoadhesive caps that are melted onto the cells of interest.

 Image of the Pixcel II LCM

 

Technical details:

 Inverted microscope

 

Lenzes:

Magn/NA: 4x/0.13,10x/0.3, 20x/0.4

 

Fluorescence filters:

FITC

HQ Texas Red

CY5

 

Contact: