Applied Flow Cytometry

Target group

Members of the Doctoral School of Life Sciences and Medicine

Level

Basic knowledge in flow cytometry

Organizers

- Prof. dr. Evelyne Meyer [] en ing. Kristel Demeyere (lab responsible) - Research group Biochemie, Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 29

- Prof. dr. Bert Devriendt - Research group Immunologie, Department of Virology, Parasitology and Immunology, Faculty of Veterinary, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 43

- Prof. dr. Karine Breckpot [Karine.Breckpot@vub.be] - Laboratory for Molecular and Cellular Therapy, Department of Biomedical Sciences, Vrije Universiteit Brussel

- Prof. dr. Stefaan Verhulst [Stefaan.Verhulst@vub.be] - Scientific Director of Flow Cytometry Core Facility, Vrije Universiteit Brussel

- Prof. dr. Nathalie Cools - Laboratory of Experimental Haematology, Vaccine & Infectious Diseases Institute (VAXINFECTIO), Universiteit Antwerpen, Universitair Ziekenhuis Antwerpen, Wilrijkstraat 10, 2650 Edegem. Tel. 03 821 3584

Programme and venue (3-days course)

Monday 26 September 2022 - 10:00-12:00 / 13:00-16:00

A. Diagnosis of leukaemia and lymphoma: flow cytometry in the diagnostics and follow-up of these diseases in humans with attention to the techniques used (morning).

B. Practical aspects: in the laboratory, explanation by the person responsible for the flow cytometer (afternoon).

  • Location: VUB: Laboratorium Hematologie, UZ Brussel, Paviljoen Louis Tielemans, niveau 0, Laarbeeklaan 101, 1090 Brussel (Entrance via the main entrance of UZ Brussel. In the hall, take the corridor at the left (direction cardiovascular diseases). Laboratory for Clinical Biology is seen in front of you.

 

Tuesday 27 September 2022 - 9:30-12:00 / 13:00- 16:00

A. Detection of selected cytokines in a murine tumor lysate using luminex kit for cytometer bead array analysis.  Practical aspects in the Biochemistry laboratory and explanation of the measurements by the person in charge at the 3-laser flow cytometer with attention to the pitfalls in multiplex bead analysis (morning).

B. Purification of 4 different immune cells (cytotoxic T cells, NK cells, monocytes and pDCs) from the PBMC fraction in the pig. Practical aspects at the Laboratory of Veterinary Immunology, explanation of sorting process by the person responsible for the cell sorter and ‘tip and tricks’ to prepare an efficient sort (afternoon).

 

Wednesday 28 September 2022 - 10:00-12:00 / 13:00-16:00

A. Detection of antigen-specific T cells by intracellular cytokine determination (ICS) (morning).

B. Practical aspects: performing protocol (fix / perm) + antibody staining, followed by flow cytometric analysis with extra attention to pitfalls in ICS (afternoon).

  • Location: UA, Lab Experimentele Hematologie, Vaccin & Infectieziekten Instituut (VAXINFECTIO), Universiteit Antwerpen, Wilrijkstraat 10 - 2650 Edegem. Tel: 03/ 821 35 84

Registration fee

Free of charge for the selected participants of the Doctoral School of Life Sciences and Medicine of Ghent University.

Registration procedure

To register please follow this link: https://eventmanager.ugent.be/AppFlowCyto  In case the course is fully booked, you can ask by e-mail to be added to the waiting list. Please mention your student ID nr.

Please read the cancellation policy: cancellation policy courses

Language

English

Number of participants

Maximum 6

Evaluation criteria (doctoral training programme)

Presence + active participation in all sessions