Workshop Applied Flow Cytometry 2019-2020 - (pre)clinical hematological analyses

Target group

Members of the Doctoral School of Life Sciences and Medicine

Level

Basic knowledge in flow cytometry

Organizers

- Prof. dr. Evelyne Meyer [] en ing. Kristel Demeyere (laboverantwoordelijke) - Onderzoeksgroep Biochemie, Vakgroep Farmacologie, Toxicologie en Biochemie, Faculteit Diergeneeskunde, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 29

- Dr. Bert Devriendt postdoctoral researcher - Onderzoeksgroep Immunologie, Vakgroep Virologie, Parasitologie en Immunologie, Faculteit Diergeneeskunde, Universiteit Gent, Salisburylaan 133, 9820 Merelbeke. Tel. 09 264 73 43

- Prof. dr. Sam Van der Meeren [], Laboratorium Hematologie, Universitair Ziekenhuis Brussel, Paviljoen Louis Tielemans, Laarbeeklaan 101, 1090 Brussel. Tel. 02 4775071

- Prof. dr. Nathalie Cools - Laboratorium Experimentele Hematologie, Vaccin & Infectieziekten Instituut (VAXINFECTIO), Universiteit Antwerpen, Universitair Ziekenhuis Antwerpen, Wilrijkstraat 10, 2650 Edegem. Tel. 03 821 3584

Programme and venue (3-days course): from Mon 23 to Wed 25 September 2019

  • Monday 23 September 2019 - 10:00-12:00 / 13:00-16:00

Diagnosis of leukaemia and lymphoma: flow cytometry in the diagnosis and follow-up of these diseases in humans with attention to the techniques used (morning).

Practical aspects: explanation by the responsible of the flowcytometer (afternoon).

  • Location: Laboratorium Hematologie, UZ Brussel, Paviljoen Louis Tielemans, level 0, Laarbeeklaan 101, 1090 Brussel (Entrance via the main entrance of UZ Brussel. In the hall, take the corridor at the left (direction cardiovascular diseases). Laboratory for Clinical Biology is seen in front of you.

 

  • Tuesday 24 September 2019 - 9:30-12:00 / 13:00- 16:00

A. Detection of selected cytokines in mice serum (submitted by preclinical infection model) using a commercial kit (Luminex) for cytometer bead array analysis. Practical aspects: explanation of the measurements on the 3 laser instrument by the responsible of the Cytoflex flow cytometer with attention to the pitfalls in multiplex bead array analysis (morning).

B. Purifying 4 different types of immune cells (cytotoxic Tcells, NK cells, monocytes and pDCs) from the PBMC fraction of porcine blood. Practical aspects: explanation of the sorting process on the instrument by the responsible of the FACSAria III celsorter including 'tips and tricks' to perform an efficient sort (afternoon).

  • Location: Ghent University, Laboratory of Biochemistry (morning) and Immunology (afternoon) Faculty of Veterinary Medicine, Salisburylaan 133- 9820 Merelbeke - High Building D1 entrance nr 24, Unit A & B - 2nd resp. rd floor. 

 

  • Wednesday 25 September 2019 - 10:00-12:00 / 13:00-16:00

Detection of antigen specific T cells using intracellular cytokine determination (ICS) (morning).
Practical aspects: performing protocol (fix/perm) + antibody staining, followed by flowcytometric analysis with attention to pitfalls at ICS (afternoon).

  • Location: UA, Laboratory of Experimental Hematology, Vaccine & Infectious Disease Institute (VAXINFECTIO), Universiteit Antwerpen, Wilrijkstraat 10 - 2650 Edegem. Route description UZA Tel: 03/ 821 35 84

Registration fee

Free of charge for the selected participants of the Doctoral School of Life Sciences and Medicine of Ghent University.

Registration procedure

To register please follow this link: https://eventmanager.ugent.be/flowcyto

Please read the cancellation policy: cancellationpolicycourses

Language

English

Number of participants

Maximum 7

Evaluation criteria (doctoral training programme)

Presence + active participation in all sessions