Guidelines PCR analysis

Guidelines

  • For starters, take a hands-on course on PCR and pipetting.
  • Think about your experiment, plan and consult to avoid unnecessary or unusable runs.
  • Buy what you need and do it together.
  • Use best practice guidelines and relevant controls.
  • Avoid optimization runs with relentless in-silico characterization of primers, probes and normalization genes.
  • Do not store your work stocks of primers and probes separately, but together in 1 ep.
  • Work in small volumes (10µl for normal reaction, 20µl for cloning), consider 384 well plates.
  • Avoid duplicates as much as possible.
  • Automation can reduce consumption of consumables.
  • Set room temperature for your last cycle.

Course

Organise a hands-on course. Information is following.

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