Fluorescence lifetime imaging microscopy of cell metabolism in organoid models

About this line of research

Using high-performance fluorescence lifetime imaging microscopy (FLIM) and phosphorescence lifetime imaging microscopy (PLIM) biosensors and approaches, we integrate them in live 3D multi-parameter assays, addressing requirements of metabolites in the medium, inflammatory responses, consequences of radiotherapy, vitamins and drug action, cell death and host-microbiota interactions in organoid systems.

Our current model is the mouse adult stem cell-derived intestinal organoids. With the multi-parameter imaging and biofabrication approaches, we address current limitations of organoid models, such as their high heterogeneity and controllable growth.

Publications

  1. *Okkelman IA, Puschhof J, Papkovsky DB, Dmitriev RI: Visualization of Stem Cell Niche by Fluorescence Lifetime Imaging Microscopy. In: Intestinal Stem Cells: Methods and Protocols. Edited by Ordóñez-Morán P. New York, NY: Springer US; 2020: 65-97.
  2. *Okkelman IA, Neto N, Papkovsky DB, Monaghan M, Dmitriev RI: A deeper understanding of intestinal organoid metabolism revealed by combining fluorescence lifetime imaging microscopy (FLIM) and extracellular flux analyses. Redox Biology 2019:101420.
  3. *Okkelman I.A., Foley T., Papkovsky DB, Dmitriev RI: Live cell imaging of mouse intestinal organoids reveals heterogeneity in their oxygenation. Biomaterials 2017, 146, 86-96.

Questions?

  • Ruslan Dmitriev, professor/head of the group

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