Infrastructure and techniques - Physiology

The available infrastructure and equipment is specialized for our core research activities, which are focused at calcium imaging, connexin channel studies, cell death communication and blood-brain barrier function.

 

Cell culture and biomolecular techniques

  • Cell culture facility: flow, incubators ...
  • Mutagenesis, bacterial transformation and transfection (L2 facility since 2014)
  • PCR and DNA gel electrophoresis
  • Western blot equipment
  • Plate reader for luminescence, fluorescence en absorbance measurements

Microscope imaging facility

  • Epifluorescence microscope equipped with cooled, high-sensitivity CCD camera’s
  • Ca2+ imaging microscope equipped for UV photolysis studies
  • Fast laser scanning microscope for FRAP studies
  • Two photon microscope for in vivo imaging
  • High throughput automized microscope imaging and analysis station (BD pathway 435)
  • Leica TCS SP8 X tunable WLL spectral confocal equipped for live cell calcium imaging and UV photoactivation studies

Electrophysiology

    • Electrophysiology patch-clamp setups for single channel unitary current studies

    Electroporation

      • Equipment for localized electroporation-based cell loading of membrane-impermeable substances including peptides and proteins