Infrastructure and techniques - Radiobiology

Biomolecular techniques

• DNA isolation from blood/buffy coat
• Genotyping techniques for single nucleotide polymorphisms : Restriction fraction length polymorphism technique (RFLP) and High resolution melting technique (HRM)
• Q-PCR, western blotting

Histological and cytogenetic techniques

• Histological, immunohistochemical and FISH staining techniques
• Cytogenetic techniques for the detection of radiation damage: micronucleus assay, dicentric assay, gamma -H2AX foci assay
• Metafer: automatic image analysis for DNA damage and repair
• Chromoscan: automatic image capturing