EV isolation from biological fluids

LECR provides a combination of technologies to isolate and characterize extracellular vesicles (EV) from complex biofluids including, but not limited to, blood, urine and cell culture medium. Methods include size-exclusion chromatography, robot-assisted density-gradient ultracentrifugation, fluorescent and non-fluorescent nanoparticle tracking analysis. Size-exclusion chromatography allows to separate EV from bulk proteins based on size separation. Density gradient ultracentrifugation (using SW 32.1 Ti rotor) separates EV from proteins based on density using discontinuous iodixanol gradient. Biomek® 4000 Liquid Handler allows full automatic gradient preparation and fraction isolation for optimal reproducibility. Nanoparticle tracking analysis (NTA) using a Nanosight LM10 is a method used to quantify and determine the size distribution and number of nanoparticles ranging from 70 nm to 1 µm of diameter. The NTA at LECR is equipped with a pump system for optimal reproducible sampling and 405nm (violet), 488nm (blue) and 532nm (green) lasers.