CAM models

The chicken Chorio-Allantoic Membrane (CAM) model, the chick embryo is surrounded by the chorioallantoic membrane (CAM), a highly vascularized extra-embryonic membrane that can be used to graft human cells. When grafted on CAM, tumor cells are capable of stimulating formation of new blood vessels, gaining their blood supply. The chick embryo is naturally immunoincompetent, thus easily allowing mammal cell xenografts. The procedures are relatively simple, involving short experimental times and low costs. The use of this alternative animal model fits in the "3Rs policy".

 

Reference: Sys G. et al., Tumor grafts derived from sarcoma patients retain tumor morphology, viability, and invasion potential and indicate disease outcomes in the chick chorioallantoic membrane model, Cancer Letters, 326, 69-78, 2012.

Video derived from JOVE.