Nano Particle Tracking

Nano Particle Tracking techniqueNanoparticle tracking analysis (NTA) is a technique routinely used for the quantification and characterization of extracellular vesicles. It uses light scattering and Brownian motion properties of particles in a sample to calculate the size distribution and concentration thereof. A laser beam is passed through a chamber containing particles in suspension and the resulting scattered light is detected, through a microscope (x20), with a video camera mounted on top. This camera then captures video files of the particles moving under Brownian motion and the NTA software then tracks each particle individually and calculates their hydrodynamic diameter using the Stokes-Einstein equation. The particle concentration is calculated based on the number of particles detected per screen and the total volume of the chamber. LECR is equipped with a LM10 NTA device provided with a 405nm laser for routine light scatter analysis and a 488nm laser for the analysis of green fluorescently labelled particles. For the latter a 500nm long pass filter is placed in front of the camera and a continuous pump system is used for avoiding photo-bleaching effects. Furthermore the LM10 NTA device is provided with a 532nm laser.

Refernce: Van Deun, J., et al., The impact of disparate isolation methods for extracellular vesicles on downstream RNA profiling. J. Extracell. Vesicles., 3, 2014. (I.F. 2014 = NA) (ranking: NA) (new journal) (citations: >185).