Protein analysis

Protein analysisThe BioDrop (Isogen Life Science) is a UV/Vis spectrophotometer for measurement of DNA, RNA, oligonucleotides and protein samples. The Qubit 3.0 (Invitrogen) is a benchtop fluorometer for measurement of DNA, RNA, oligonucleotide and protein quantity or quality. Because this technique makes use of fluorescence it is more sensitive than UV absorbance-based quantification. The lab is fully equipped to perform SDS-PAGE and Western blot analysis. In this way proteins can be separated on the basis of their molecular weight and further analysed by an immunostaining. Imaging of the blot is performed by the Proxima 2850 Imager (Isogen Life Science). The Proxima can also be used for imaging of ethidium bromide stained DNA agarose gels, coomassie stained gels, SYBR Green stained gels, petri dishes, etc.

 

 

Reference: Vergauwen, G., et al., Confounding factors of ultrafiltration and protein analysis in extracellular vesicle research. Scientific Reports, (2017), 7(1), 12.