Scaffolds

We make use of 3D printed gelatin coated PLA scaffolds (d 6mm, h 4mm) to co-culture cells in a 3D manner. The cells are suspended in a collagen gel and transferred to the scaffolds by applying vacuumed pressure. During in vitro culture, the cells self-organize themselves and form 3D structures. These scaffolds can also be implanted in an animal model. Within 4 weeks the scaffold is infiltrated by host- tissue and supplied by newly formed blood vessels, visible on contrast enhanced µCT.

Left Confocal image of a scaffold seeded with 2 × 106 SK-OV-3 Luc eGFP (green) and 8 × 106 CAF tdTomato (red) after 5 weeks of in vitro culture. Inset shows phase-contrast image at similar position of confocal image. Dotted line indicates spheroid in both confocal and DIC image. Right H&E staining of a tumor scaffold 4 weeks post implantation. Black rectangular boxes on the overview slide indicate the areas used for magnification represented in 1–4. PLA; PLA scaffold that is dissolved during histological processing. S; Spheroid of cancer cells and CAF. B; Blood vessel. Me; Mesothelium. Mu; Muscle. St; Stroma. Ic; Immune cells. At; Adipose tissueLeft: Confocal image of a scaffold seeded with 2 × 106 SK-OV-3 Luc eGFP (green) and 8 × 106 CAF tdTomato (red) after 5 weeks of in vitro culture. Inset shows phase-contrast image at similar position of confocal image. Dotted line indicates spheroid in both confocal and DIC image.

Right: H&E staining of a tumor scaffold 4 weeks post implantation. Black rectangular boxes on the overview slide indicate the areas used for magnification represented in 1–4. PLA; PLA scaffold that is dissolved during histological processing. S; Spheroid of cancer cells and CAF. B; Blood vessel. Me; Mesothelium. Mu; Muscle. St; Stroma. Ic; Immune cells. At; Adipose tissue

Reference: De Jaeghere E., et al., Heterocellular 3D scaffolds as biomimetic to recapitulate the tumor microenvironment of peritoneal metastases in vitro and in vivo. Biomaterials, Biomaterials (I.F. 2016 = 8.402) (Ranking : materials science, biomaterials 1/33)