Program:

12.00 – 12.05: Welcome by Prof. Kevin Braeckmans

12.05 – 12.10: Introduction to GLiM by dr. Herlinde De Keersmaecker

12.10 – 12.45: Principles and applications of Two-Photon Microscopy by Dr. Herman Fennema (Nikon)

12.45 – 13.00: Q&A

13.00 – 14.00: Sandwich lunch

14.00 – 17.00: Demonstrations at the microscope (45 min)

Register before 17 April 2023

What is two-photon excitation Microscopy?

In two-pohton excitation (2PE) microscopy, simultaneous absorption of two photons is used to excite fluorescent molecules. For this excitation process light in the near infrared (NIR) range of the spectra is used instead of the visible range. The use of NIR light leads to the capability of deeper tissue penetration in scattering samples compared to 1 photon excitation as is used for confocal microscopy. Moreover, 2PE microscopy has intrinsic capabilities to perform optical sectionning thereby allowing the imaging of three dimensional structures of biological samples. 

More background information can be found  here.