Confocal & multi-photon microscopes

Overview of confocal microscopes available at the expertise centre and their unique features:

Nikon A1R HD confocal & multiphoton microscope

Instrument specificationsNikon A1R multiphoton

  • 4 lasers (405 nm, 488 nm, 561 nm, 640 nm)
  • Galvoscanner and resonantscanner
  • Perfect Focus System
  • 4 detection channels (2 GaASP and 2PMT detectors) with choice of different emission filters
  • 32-channel spectral detector unit
  • Incubator with temperature regulation at the microscope level and CO2 at the cell culture level
  • Multi-photon laser (Mai Tai TI:SAPPHIRE laser, 690-1040 nm)
  • Non-descanned detectors
  • Objective lenses:
    • 60X water objective with automated correction ring (SR plan apo IR 60X WI, NA 1.3, WD 180µm)
    • 60X oil (plan apo λ 60X oil, NA 1.4, WD 130 µm)
    • 40X air (plan apo λ 40X, NA 0.9, WD 250 µm)
    • 20X air (plan apo VC 20X, NA 0.8, WD 1000 µm)

Contact us for more information

Location

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

Price

Price category 3

Price category 4  (Multiphoton)

Nikon A1R confocal microscope

Instrument specificationsAR1.png

  • 4 lasers with in total 7 laser lines (405 nm, 457 nm, 476 nm, 488 nm, 514 nm, 561 nm, 639 nm)
  • Galvoscanner and resonantscanner. Simultaneous use possible for photo-stimulation experiments (FRAP, FRET, ...)
  • Piezo stage for fast and precise scanning in the z direction
  • Perfect Focus System
  • Simultaneous 4-channel PMT detection with choice of different emission filters
  • 32-channel spectral PMT detector, useful for spectral imaging
  • 2 separated lasers for Controlled Light Exposure Microscopy
  • Incubator with temperature regulation at the microscope level and CO2 at the cell culture level

Contact us for more information

Location

Faculty Bioscience Engineering,

Department of Biotechnology,

Coupure Links 653, Blok A,

B-9000, Ghent

Price

Price category 2

Nikon C2 confocal microscope

Instrument specificationsC1.png

  • 4 Laser lines (405 nm, 488 nm, 560 nm, 637 nm)
  • 3 PMT detectors and spectral detector, useful for spectral unmixing
  • Photo-stimulation
  • Fluorescence correlation spectroscopy (FCS) module

Contact us for more information

Location

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

Price

Price category 2

Leica TCS-SP8 confocal microscope

Instrument specificationsleica.png

  • White light laser (470 – 670 nm), up to 8 freely tunable laser lines and 2 UV Laser lines (355 nm, 405 nm)
  • 2 HyD (sensitive detection) and 2 PMT detectors
  • Flexible tuning of emission bands via acousto-optical beam splitter (AOBS)
  • Possibility of spectral imaging, (UV) photo-stimulation and resonant scanner
  • Live cell imaging: Temperature and CO2 incubator

Contact us for more information

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent

Price

Price category 3