Confocal & multi-photon microscopes

Overview of confocal microscopes available at the expertise centre and their unique features:

Nikon A1R HD confocal & multiphoton microscope

Instrument specifications

4 lasers (405 nm, 488 nm, 561 nm, 640 nm)
Galvoscanner and resonantscanner
Perfect Focus System
4 detection channels (2 GaASP and 2PMT detectors) with choice of different emission filters
32-channel spectral detector unit
Incubator with temperature regulation at the microscope level and CO₂ at the cell culture level
Multi-photon laser (Mai Tai TI:SAPPHIRE laser, 690-1040 nm)
Non-descanned detectors
Objective lenses:
- 60X water objective with automated correction ring (SR plan apo IR 60X WI, NA 1.3, WD 180µm)
- 60X oil (plan apo λ 60X oil, NA 1.4, WD 130 µm)
- 40X air (plan apo λ 40X, NA 0.9, WD 250 µm)
- 20X air (plan apo VC 20X, NA 0.8, WD 1000 µm)

Location

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

Price

Price category 3

Price category 4 (Multiphoton)

Nikon A1R confocal microscope

Instrument specifications

4 lasers with in total 7 laser lines (405 nm, 457 nm, 476 nm, 488 nm, 514 nm, 561 nm, 639 nm)
Galvoscanner and resonantscanner. Simultaneous use possible for photo-stimulation experiments (FRAP, FRET, ...)
Piezo stage for fast and precise scanning in the z direction
Perfect Focus System
Simultaneous 4-channel PMT detection with choice of different emission filters
filters for epifluorescence detection:
- empty
- Analyzer
- DAPI (Ex. 377/50, Em. 447/61)
- GFP (Ex. 472/30, Em. 520/35)
- TxRed (Ex. 562/40, Em. 642/40)
- YFP (Ex. 500/20, Em. 535/30)
- 32-channel spectral PMT detector, useful for spectral imaging
2 separated lasers for Controlled Light Exposure Microscopy
Incubator with temperature regulation at the microscope level and CO₂ at the cell culture level
Objective lenses:
- 10X plan apo AIR dic N1(NA 0,45; WD 4; brightness 4,10) MRD70170
- 20X plan fluor AIR dic N2 (NA 0,5; WD 2,1; brightness 1,56) MRH00205
- 40X S plan fluor ELWD AIR (NA 0,6; WD 3,6-2,8; brightness 0,81) MRH08430
- 40X plan fluor OIL dic H/N2 (NA 1,3; WD 0.2; brightness 17,85) MRH01401
- 60X plan apo VC OIL dic N2 (NA 1,4; WD 0.130; brightness 10,67)
- 60X plan apo VC H2O dic N2 (NA 1,2; WD 0,31-0,28; brightness 5,76) MRD07602

Location

Faculty Bioscience Engineering,

Vakgroep Biotechnologie, BW25

Campus Proeftuin, Gebouw N1, Lokaal 36.01.100.030

B-9000, Ghent

Price

Price category 2

Leica TCS-SP8 confocal microscope

Instrument specifications

White light laser (470 – 670 nm), up to 8 freely tunable laser lines and 2 UV Laser lines (355 nm, 405 nm)
2 HyD (sensitive detection) and 2 PMT detectors
Flexible tuning of emission bands via acousto-optical beam splitter (AOBS)
Objectives:
- HC FLUOTAR L 25x/0,95 W VISIR (#506375)
- HC PL APO 40x/1,30 OIL PH3 CS2 (#506359)
- HC PL APO 63x1,2 W CORR UVIS CS2 (#506355)
Possibility of spectral imaging, (UV) photo-stimulation and resonant scanner
Live cell imaging: Temperature and CO₂ incubator

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Building The CORE, 3th floor

B-9000, Ghent

Price

Price category 3

Leica Stellaris 8 Falcon FLIM microscope

Instrument specifications

FALCON: FLIM image acquisition, processing and analysis
Objectives:
- HC PL APO 10x/0.40 CS2
- HC FLUOTAR L 25x/0,95 W VISIR (with automated water dispenser)
- HC PL APO 40x/1.25 GLYC CORR CS2
- HC PL APO 63x/1.40 OIL CS2
Excitation:
- White light laser (440 – 790 nm), up to 8 freely tunable laser lines
- 405 pulsed + CW laser
- Pulse picker (Pulse frequency 78, 39, 20, 10, 5, 2.5MHz)
Detectors:
- pos 2: HyD X > 46% @ 500 nm, 410-750 nm, digital and counting
- pos 3: HyD S > 58% @ 500 nm, 410 – 850 nm, analog and counting
- pos 4: HyD X > 46% @ 500 nm, 410-750 nm, digital and counting
- pos 5: HyD R> 26% @ 635 nm, 410-830 nm, digital and counting (enhanced detection efficiency in NIR)
Spectral Detection: 410 – 850 nm, fully tunable emission bands
Galvano and 8kHz Resonant scanner
Live cell imaging: Temperature and CO₂ incubator
sample holders:
- universal insert (standard Ibidi or glass slides, 35 mm dishes (glass bottom)
- insert for 96 well plate
DMi8 CS body with:
- auto focus correction (AFC)
- automated scanning stage
- filtercubes for epifluorescence observation (DAPI, FITC, RHOD)
LAS X STELLARIS Control Software with 3D visualisation and navigator
Lightning (for deconvolution) an DSE Dynamic Signal Enhancement (rolling averaging for fast acquisition)

Location

Faculty of Medicine and Health Sciences,

Department of Human structure and repair,

Tissue Engineering and Biomaterials Group

C. Heymanslaan 10, Building The CORE, 1st floor (animal facility)

B-9000, Ghent

Price

The pricing is subject to the grant used to acquire the instrument. Contact us for more information.

Zeiss LSM900 (G-FaST group)

Instrument specifications

Objectives:
- Fluar 5x/0.25 WD=12.5 M27
- Plan-Neofluar 20x/0.50
- Plan-Apochromat 40x/1.3 Oil DIC UVVIS-IR (oil: Zeiss, Immersol 518F, cat. nr. 444960-0000-000)
- Plan-Apochromat 63x/1.40 Oil DIC (oil: Zeiss, Immersol 518F, cat. nr. 444960-0000-000)
Excitation:
- Diode laser 405nm, 5mW
- Diode laser 488nm, 10mW
- Diode laser (SHG) 561nm, 10mW
- Diode laser 640nm, 5mW
Detectors:
- 2 Multi Alkali PMT's
- Spectral Detection:tunable emission bands
Axio Observer 7 stand for LSM 900:
- Transmitted Light DIC
- motorized scanning stage
- filtercubes for epifluorescence observation
  - DAPI (Filterset 49 DAPI shift free)
  - GFP (Filterset 38 Endow GFP Shift-free)
  - Cy3 (Filterset 43 Cy3, d=25 shift free)
Galvano scanner
Live cell imaging: Temperature and CO₂ incubator
sample holders:
- universal insert (standard Ibidi or glass slides, 35 mm dishes (glass bottom)
- insert for 96 well plate
Zen Blue & Black Software

Location

Faculty of Medicine and Health Sciences,

Department of Human structure and repair,

Ghent Fertility and Stem Cell Team (G-FaST)

C. Heymanslaan 10, Building The CORE, 1st floor (animal facility)

B-9000, Ghent

Price

Price category 2