Laser capture microscopes

Overview of laser capture microscopes available at the expertise centre and their features:

Zeiss PALM microbeam (Merelbeke)

Instrument specifications

Laser Capture Microdissection

  • Inverted Axiovert 200 Zeiss microscope
  • Objectives: 5X, 10X, 20X, 40X, 63X air and 100X oil
  • Four colour channels for epifluorescence visualisation (HXP 120)
    • DAPI (ex. G 365 /em. BP 445-50)
    • FITC (ex. BP 470-40 / em. BP 525-50)
    • AF555 (ex. BP 546-12 / em. BP 575-640)
    • SpectrumAqua (ex. BP 436-20 / em. BP 480-40)
  • Two detectors: Axiocam 506 mono and Axiocam 506 color
  • Pulsed UVa-laser for sample collection
  • PALM RoboSoftware
  • ApoTome for optical sectioning (not combined with LCM operation)
  • Stage top Incubator (temperature control)
  • RoboMover
  • Multiple inserts (200 or 500 µl tubes to load load either a single cap, eight caps, up to 12 eight cap strips or a 96 well plate)
  • Live cell collector

Contact us for more information

Location

Faculty Veterinary Medicine,

Department of Morphology,

Salisburylaan 133,

B-9820, Merelbeke

Price

Price category 1

Zeiss PALM microbeam (Heymans)

Instrument specifications

Zeiss PALM microbeam laser capture microscope

  • Inverted Axiovert 200 Zeiss microscope
  • Objectives:
    • 10X air, Fluor, 0.5NA
    • 20X air, LD Plan-Neofluar 0.4 NA
    • 40X air, LD Plan-Neofluar 0.6 NA
    • 63X air, LD Plan-Neofluar 0.75 NA
    • 63X oil, plan Apochromat, 1.4 NA
    • 100X oil, EC Plan-Neofluar, 1.3 NA IRIS
  • Colour channels for epifluorescence visualisation (HXP120)
    • Rhodamine, GFP, CFP, DAPI, B/G/HeRed
  • Two detectors: Axiocam 506 mono and Axiocam 503 color
  • Pulsed UVa-laser for sample collection
  • PALM RoboSoftware
  • ApoTome for optical sectioning (not combined with LCM operation)
  • CapMover
  • Inserts  for 200 µl and 500 µl tubes to load load either a single cap or two caps

Contact us for more information

Location

Faculty of Pharmaceutical Sciences,

Department of Pharmaceutics,

Ottergemsesteenweg 460,

B-9000, Ghent

Price

Price category 1