Official opening: Laser Capture Microdissection

(27-09-2019) Festive announcement of the availability of two high-end Laser Capture Microdissection instruments.

Invitation


We are glad to invite you on the festive official opening of two high-end laser capture microdissection instruments, part of the Centre for Advanced Light Microscopy.

 

When: 21 October 2019, 12.00h
Where: Auditorium D, 72.04 - D4 Klinieken, Salisburylaan 133, 9820 Merelbeke
Program:
12.00 – 12.05: Official announcement by Prof. Ward De Spiegelaere
12.05 – 12.15: Centre for Advanced Light Microscopy by dr. Herlinde De Keersmaecker
12.15 – 12.45: Possibilities and expertise on laser capture microdissection by Prof. Ward De Spiegelaere and Jana Weymaere
12.45 – 13.45: Tour and demonstration
During this program we offer you a sandwich lunch.


Register here

What is laser capture microdissection?LCM expertise


Laser capture microdissection (LCM) is a method for isolating specific cells of interest from microscopic regions of tissue/cells/organisms (dissection on a microscopic scale with the help of a laser). LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations. The isolated samples can be used for e.g. gene expression analysis with RT-qPCR, sequencing or protein analysis by mass spectrometry. In LCM, the sample is first visualised by light or fluorescence microscopy to find a region of interest. This region of interest is subsequently cut out of the sample using a powerful UV laser beam. Thereafter the dissected region is collected in a non-contact way to prevent any contamination by catapulting the sample using a short UVa laser pulse.


Advantages of laser capture microdissection

  • Obtaining pure cell populations without the risk of contamination
  • Genome, transcriptome and proteome analysis after isolation possible
  • Can be performed on different sample types such as snap frozen or formalin fixed paraffin-embedded tissue sections, cytospins, cell smears, chromosome preparations and live cells.
  • Can be combined with fluorescence staining to visualize particular cells or cell organelles of interest