Nanoscopy-on-a-chip

For whom
Students , Employees , Alumni
When
08-01-2021 from 11:30 to 12:30
Where
online via Teams
Language
English
Organizer
Kamal Kaur
Contact
kamalpreet.kaur@ugent.be
Website
https://eventmanager.ugent.be/seminarBSA
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NB-Photonics seminar by prof. Balpreet Singh Ahluwalia from University of Tromsø, The Arctic University of Norway on "Nanoscopy-on-a-chip"

Abstract

Super-resolution optical microscopy commonly referred as optical nanoscopy has provided a glimpse of its future impact on biology and life science. Although optical nanoscopy offers unprecedented opportunities, the vision to replace present day standard microscopes with next generation super-resolution microscopy is still far from reality due to present limitations of nanoscopy methodologies. The large-scale adoption of optical nanoscopy is currently hindered by system complexity, cost, lack of multi-modality, imaging speed and the need for skilful and highly trained operators. Moreover, the throughput of present-day nanoscopy methods is limited to study statistically relevant biological problems.

Photonic integrated circuits (PIC) reduce the footprint, cost and complexity of optical systems. PIC technology enables on-chip integration of several optical functions. The compatibility with standard optical fibre components enables high-speed light coupling into PICs. Here, I will provide an overview of several optical nanoscopy methodologies developed that we have developed using an integrated photonic-chip. We have developed photonic-chip based single molecule localization optical microscopy (on-chip SMLM) , on-chip structured illumination microscopy (on-chip SIM) , light intensity fluctuation based optical nanoscopy and correlative light-electron microscopy . Using the principle of single molecule localization, we demonstrate a resolution of 50 nm using chip-based nanoscopy. Furthermore, we demonstrate the capability of chip-based nanoscopy to acquire super-resolved images over millimetre field-of-view scale; a 100-fold increase in imaging area as compared to other nanoscopy solutions, thus opening the opportunities of high-throughput optical nanoscopy.

Bio-Sketch

Professor Balpreet Singh Ahluwalia is working at the Department of Physics & Technology, UiT The Arctic University of Norway. Ahluwalia is also affiliated as visiting scientist at the Department of Clinical Sciences, Intervention and Technology (CLINTEC), Karolinska Institute in Sweden. Previously, Ahluwalia have been visiting scientist/professors at the Centre of Biophotonics of Science and Technology, University of California Davis (2012) and at the Opto-electronics Research Centre (ORC), University of Southampton (2008, 2019). Ahluwalia leads Optical Nanoscopy Research Group, which is driven by curiosity to image the nanoscale biological systems, by constantly pushing the fronteirs in advanced microscopy and translating its impact on biological and clinical applications. Ahluwalia research interest comprises in integrated optics, optical nanoscopy, nano-fabrication and cell biology. Ahluwalia is a receipt of several European Union and Norwegian Research Council projects including 3 ERC projects (1 ERC St. Grant + 2 PoC) in the areas related to nano-photonics, optical sensors and optical nanoscopy. Ahluwalia is the inventor of chip-based optical nanoscopy, holds several patents in this field and is co-founder of Chip NanoImaging AS (www.chipnano.com). As a recognition of his contribution to the field of optics and advanced microscopy, Ahluwalia was awarded 2018 Tycho Jæger Prize in Electro-optics and 2018 University’s Research & Development Award.