Seminar by Prof. Wolfgang Langbein on "Coherent Raman microscopy"

For whom
Students, Employees, Alumni, Press
29-08-2019 from 15:30 to 16:30
iGent, Technologiepark Zwijnaarde 126, 9052
Kamal Kaur
Add to my calendar


Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Coherent Raman Scattering (CRS) microscopy has attracted increasing attention as a powerful multiphoton microscopy technique which overcomes the need of fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution [1]. Over the past 10 years, our laboratory has developed and demonstrated a range of label-free CRS microscope set-ups featuring innovative excitation/detection schemes and quantitative image analysis. Using hyperspectral imaging [2] and associated quantitative chemical imaging algorithms and unsupervised analysis [3], we have determined the lipid uptake of fixed and living adipose derived human stem cells differentiating into pre-adipocytes [4], the lipid content and spatial distribution in live mammalian oocytes and early embryos [5], addressed drug-induced lipid storage within hepatic tissue [6], and quantitatively measured masses of lipids, proteins and DNA during cell division [7]. We have also shown that CARS can be used to visualise single non-fluorescing nanodiamonds in cells [8] and developed [9] epi-detected heterodyne CARS (eH-CARS) imaging of individual lipid bilayers. I will present our latest progress with these techniques and their applications to bio-imaging.



Prof. Wolfgang Langbein received his Diploma in physics from the University of Kaiserslautern in 1992, and his PhD in physics from the University of Karlsruhe in 1995. From 1995 to 1998, he was assistant research professor at the Mikroelektronik Centret, Denmark. From 1998 to 2004, he was with the University of Dortmund, where he received his Habilitation in 2003. In 2004 he was appointed Senior Lecturer in the School of Physics, Cardiff University, promoted to Reader in 2006 and to Personal Chair in 2007. One of his current research interests is the application of optical spectroscopy to life-science, focussing on the techniques of coherent anti-stokes Raman scattering (CARS) microscopy, fluorescent resonant transfer, label-free optical biosensors using microcavities, and statistical analysis of individual quantum emitters such as colloidal quantum dots.