Vitamin B1 monitoring: a micro nutrient with macro challenges.

Promovendus/a
Verstraete, Jana
Faculteit
Faculteit Farmaceutische Wetenschappen
Vakgroep
Vakgroep Bioanalyse
Curriculum
Master in Geneesmiddelenontwikkeling, Universiteit Gent (Magna cum laude), 2014-2016; Bachelor in Farmaceutische Wetenschappen, Universiteit Gent (Cum laude), 2011-2014; Wetenschappen-Wiskunde, Klein Seminarie Roeselare, 2005-2011.
Academische graad
Doctor in de farmaceutische wetenschappen
Taal proefschrift
Engels
Vertaling titel
Vitamine B1 monitoring: een micronutriënt met MACRO uitdagingen.
Promotor(en)
Prof. dr., Christophe Stove, UGent-Bioanalyse – Prof. dr., Dominique Van Der Straeten, UGent-Faculteit Wetenschappen
Examencommissie
Prof. dr., Bart De Spiegeleer, UGent-Farmaceutische Analyse – Prof. dr., Sarah De Saeger, UGent-Bioanalyse - Prof. dr., Alain Verstraete, UGent-UZGent - Prof. dr., Siska Croubels, UGent-Faculteit Diergeneeskunde - Dr., Lisbeth Patteet, Algemeen Medisch Laboratorium - Prof. dr., Bruno De Meulenaer, UGent-Faculteit Bio-ingenieurswetenschappen

Korte beschrijving

Thiamine deficiency, how to improve its prevention and diagnosis? Biofortification has been stated as a cost-effective strategy to enhance thiamine intake in developing countries, where populations are at higher risk for thiamine deficiency since they often depend on rice, containing low thiamine levels, for their main energy supply. Throughout this work, liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods were developed, allowing the determination of both the intermediate and end products of thiamine biosynthesis in plants. Arabidopsis thaliana was studied to obtain more insight into the regulation of thiamine biosynthesis, which may allow to set up rational engineering strategies to enhance the thiamine content of major food crops such as rice. The rice-specific method allowed us to steer and evaluate the effect of ongoing biofortification strategies on final thiamine levels in rice. Although LC-MS/MS is a highly sensitive and specific technique, it also involves a substantial cost and expertise, which may hamper its application in remote regions. Therefore, we offered an alternative yeast-based strategy to screen plant material and evaluate biofortification approaches. Microsampling may overcome several challenges related to the complicated venous blood collection, required for the diagnosis of thiamine deficiency, in remote regions. Therefore, we developed an LC-MS/MS method to determine thiamine diphosphate, as a marker of the thiamine status in volumetrically collected dried blood microsamples, to simplify the diagnosis of thiamine deficiency. In a large comparison study, performed on 50 healthy volunteers, we demonstrated the suitability of this alternative, patient-centric sampling approach to reliably derive the thiamine status from only one drop of finger prick blood. To conclude, the developed tools throughout this work will give rise to new opportunities in thiamine research and will aid in the fight against thiamine deficiency.

Praktisch

Datum
Donderdag 17 juni 2021, 18:00
Locatie
Faculteit Farmaceutische Wetenschappen, Auditorium B - Andreas Vesalius, Ottergemsesteenweg 460, 9000 Gent

Aangezien de UGent opnieuw on-campus doctoraatsverdedigingen toelaat, mits beschermende maatregelen (afstand houden, dragen van mondmasker, met een beperking op het aantal aanwezigen), vragen we vriendelijk om Jana te verwittigen op jrverstr.Verstraete@UGent.be indien je de verdediging wenst bij te wonen.