Innovations in rapid Mycoplasma bovis diagnostics with MALDI-TOF MS and nanopore sequencing

Bokma, Jade
Faculteit Diergeneeskunde
Vakgroep Heelkunde en Anesthesie van de Huisdieren
Jade Bokma was born on 14 August 1991 in Tegelen, the Netherlands. After finishing high school at the Alfa College in Groningen, and one year of Psychobiology at the University of Amsterdam, she started studying Veterinary Medicine at the University of Antwerp in 2011. After her bachelor’s degree she went on to study at Ghent University, where she obtained her master’s degree in Veterinary Medicine, option Ruminants, graduating summa cum laude in 2017. Her master’s thesis (“Risk factors for antimicrobial use in veal calves”) was awarded as the best master thesis on antimicrobial resistance by AMCRA (Antimicrobial Consumption and Resistance in Animals). Immediately after graduation, Jade Bokma started her PhD research at the Department of Large Animal Internal Medicine (Faculty of Veterinary Medicine, Ghent University) financed by the Belgian Federal Public Service, Health, Food Chain Safety and Environment (FOD, RF 17/6313, MALDIRESP/MA), which was supervised by Prof. dr. B. Pardon, Dr. F. Boyen, Prof. dr. P. Deprez and Prof. dr. F. Haesebrouck. This research project focused on rapid antimicrobial susceptibility testing using MALDI-TOF MS and nanopore sequencing in bacterial pathogens from the bovine respiratory tract. In addition, she was involved in a Veepeiler project (Animal Health Care Flanders) concerning Mycoplasma bovis in Flanders. She participated in the day and night shifts as veterinarian in the clinic for ruminants, took care of the analysis of clinical samples, and contributed to the education of master students. She also obtained the certificate of the Doctoral Training Programme of Life Sciences and Medicine. Jade Bokma is (co-)author of several papers in (inter)national peer-reviewed journals and professional literature. She is also reviewer for international journals, and gave multiple presentations at (inter)national conferences.
Academische graad
Doctor in de diergeneeskundige wetenschappen
Taal proefschrift
Prof. dr. B. Pardon, Faculty of Veterinary Medicine, Ghent University - Dr. F. Boyen, Faculty of Veterinary Medicine, Ghent University - Prof. dr. F. Haesebrouck Faculty of Veterinary Medicine, Ghent University
Prof. dr. E. Claerebout, Head of the jury, Ghent University - Dr. F. Tardy, UMR Mycoplasmoses des Ruminants, ANSES, Lyon, France - Dr. A. Heuvelink, Royal GD, Animal Health, Deventer, the Netherlands - Prof. dr. S. Piepers, Faculty of Veterinary Medicine, Ghent University - Prof. dr. D. Maes, Faculty of Veterinary Medicine, Ghent University, - Dr. S. Theuns, Faculty of Veterinary Medicine, Ghent University - PathoSense, Lier, Belgium

Korte beschrijving

Mycoplasma bovis is a leading, primary cause of pneumonia, arthritis, otitis and mastitis in cattle, resulting in impaired animal welfare and huge economic losses in all cattle sectors worldwide. This small bacterium lost its cell wall and several physiological mechanisms through evolution, whereupon it acquired inherent resistance against many conventional antimicrobials (e.g. penicillines, cephalosporines, sulfonamides, ..). Next to this natural resistance, M. bovis may acquire resistance against other antimicrobials as well. Currently, isolation and identification of M. bovis by culture takes 1-2 weeks, and subsequent antimicrobial susceptibility testing is currently not performed in routine diagnostics. No standard protocol is available and the lack of clinical breakpoints limits the translation of in vitro results to clinical outcome predictions of antimicrobial treatment. At a higher price, faster identification is possible with PCR (2 days). Although diagnostic accuracy of PCR is expected to be higher than culture, scientific information on this matter is limited.


Woensdag 23 juni 2021, 17:00
Diergeneeskunde Aud Maximum, Salisburylaan 133, 9820 Merelbeke

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