Spinning disk microscopesOverview of spinning disk microscopes available at the expertise centrehttps://www.ugent.be/plone_portal/glim/en/equipment/spinning-disk.htmhttps://www.ugent.be/plone_portal/++resource++plone-logo.svg
Spinning disk microscopes
Overview of spinning disk microscopes available at the expertise centre
Nikon spinning disk CSU-X1
Instrument specifications
Inverted microscope (Optional: inverter arm for upright microscopy)
Optimized for 100X Oil objective lens (Nyquist resolution)
Andor EMCCD camera (sensitive detection)
Laser lines (405 nm, 488 nm, 561 nm, 640 nm)
Frame rates up to 30 fps possible
Perfect Focus System
Live cell imaging: Temperature and CO2 (stage incubator and objective heater)
Objectives
20X: CFI Plan Apochromat Lambda D 20X, NA 0.8, WD 0.8, AIR (MRD70270)
40X: CFI Plan Fluor 40X, NA 0.75, WD 0.66, AIR (MRH00405)
40X: CFI Plan Apochromat Lambda D 40X, NA 0.95, WD 0.21 (MRD70470)
60X WI: CFI Plan Apo VC 60XC WI, NA 1.2, WD 0.31-0.28 (MRD07602)
60X Oil: CFI Plan Apochromat Lambda D 60X Oil, NA 1.42, WD 0.15 (MRD71670)
100X oil: CFI HP Plan Apo VC 100X Oil NA 1.4, WD 0.13 (MRD01902)
Emission filters:
Quad band filter: (440/40, 521/21, 607/34, 700/45)
Single band filters: 447/60, 525/30, 607/36 or 685/40nm