In sacco-in vitro rumen bypass
Assessment of rumen bypass and post-ruminal digestion (in sacco -in vitro)
Key highlights
- Exposure of test products to rumen microbial inoculum in mini nylon bags
- Resistance assessment of test products against microbial degradation
- Post-ruminal digestion simulation using mini nylon bag residues after rumen exposure
- Optimised for testing encapsulated products relying on fat- or protein-based coatings
- Customizable and comprehensive analysis to meet diverse research requirements
Examples of possible research questions
- Rumen bypass testing: evaluate how well (processed) feedstuffs or encapsulated/coated test products withstand breakdown by rumen microbes.
- Post-ruminal release: study the release and bioavailability of rumen bypass products after they have passed through the rumen environment.
Research endpoints
Rumen microbial activity analysis
- Total gas production and gas composition
- Short-chain fatty acid production
- Ammonium or lactate accumulation
- Long-chain fatty acids including biohydrogenation intermediates
- pH of rumen liquid
Analysis of nylon bag residues, e.g.
- micro-Kjeldahl analysis
- amino acids
- fatty acids, …
- Analysis of products released from the nylon bags in the incubation medium (after rumen or post-ruminal simulation)
- Delivery of the nylon bag residue or incubation medium for further analysis by customer
Customized project-specific solutions
We offer tailored experimental setup & analyses and are flexible to meet your unique research needs