Batch rumen simulation

Key highlights

• High-throughput setup with small-volume reactors for efficient multi-product screening
• Acute response testing of bioactive compounds within a simulated rumen environment.
• Impact on metabolic processes of rumen microbes by test products
• Impact of the rumen microbial activity on the test product (e.g. microbial breakdown)
• Customised experimental setup in terms of incubation time, kinetics, inoculum, …

Examples of possible research questions

• Acute dose-response of test products on enteric methane production, microbial biodegradation and conversions
• Microbial composition: study the effect of test products on microbial community structure
• Microbial breakdown of toxins (e.g. mycotoxins)

Research endpoints

Microbial (composition) analysis

• Nucleotide base analysis
• qPCR
• Metatoxonomic analysis (e.g. based on 16S rRNA, 18S rRNA, mcrA)
• Metagenomic analysis

Microbial activity analysis

• Total gas production and gas composition
• Short-chain fatty acid production
• Ammonium or lactate accumulation
• Long-chain fatty acids including biohydrogenation intermediates
• pH of rumen liquid

Customized project-specific solutions

We offer tailored experimental setup & analyses and are flexible to meet your unique research needs