Consecutive batch cultures
Consecutive batch rumen simulations
Key highlights
- Extended incubation cycle over a 2-week period to assess dynamic changes
- Serial transfers to extend the incubation cycle, enhancing longitudinal adaptation studies
- Assessment of sustained effects of test products on microbial communities and activity
- Enhanced experimental capacity compared with continuous culture methods
- Microbial enrichment through consecutive exposure to enhance anaerobic digestion of resilient substrates
- Customizable and comprehensive analysis to meet diverse research requirements
Examples of possible research questions
- Long-term dose-response: investigate the prolonged effects of test products on enteric methane production and microbial biodegradation.
- Microbial composition impact: assess the long-term effect of test products on the microbial community structure within the rumen.
- Microbial adaptation: study how the microbial population adapts to ongoing exposure to test products over an extended period.
- Microbial enrichment: selectively enrich specific microbial groups to create inocula with enhanced ability to digest resilient substrates in anaerobic environments.
Research endpoints
Microbial (composition) analysis
- Nucleotide base analysis
- qPCR
- Metatoxonomic analysis (e.g. based on 16S rRNA, 18S rRNA, mcrA)
- Metagenomic analysis
Microbial activity analysis
- Total gas production and gas composition
- Short-chain fatty acid production
- Ammonium or lactate accumulation
- Long-chain fatty acids including biohydrogenation intermediates
- pH of rumen liqui