The Incucyte® ZOOM system (Essen Bioscience) is a live-cell imaging and analysis platform that enables automated quantification of cell behavior over time. The system has high definition phase contrast optics and software recognition allowing to mask, quantify and generate time-based curves of cellular activities such as, but not limited to, 2D and 3D growth, migration (wound healing and chemotactic), aggregation (sphere formation), invasion. In addition, fluorescence tracking allows complex co-cultures conditions, phagocytosis assays, cell viability and apoptosis monitoring. The main advantage is the kinetic information which is impossible to achieve using end-point assays.
Confluence grade (phase contrast) over time of proliferating cancer-associated fibroblasts treated with a kinase inhibitor
Number of cells (nuclei) over time of proliferating cancer-associated fibroblasts treated with a kinase inhibitor
Chemotaxis of Nuclight red HT1080 cells towards different concentrations of chemoattractant (0.1%, 5%, and 10% fetal bovine serum). Confluence grade (phase contrast) of bottom view images taken by incucyte.
Chemotaxis of Nuclight red HT1080 cells towards different concentrations of chemoattractant (0.1%, 5%, and 10% fetal bovine serum). Number of cells (nuclei) of bottom view images taken by incucyte.
xCELLigence®
The xCELLigence® Real-Time Cell Analysis (RTCA) DP system (Acea Biosciences) is a live-cell monitoring platform based on label-free impedance measurements. The technology enables the continuous detection and relative quantification, expressed as a Cell Index, of spreading, growing, migrating or invading cells in real-time, without interventions of fixing and staining. Post-xCELLigence end-point measurements are possible and allow one to translate the dimensionless Cell Index parameter of the last measured time point into morphometric/colorimetric/fluorescence/luminescence data. The system has three 16-well stations allowing the use of Cell Invasion and Migration (CIM)-plates and E-plates.
