Ghent Light Microscopy CORE

Custom-build microscopes

Overview of custom-build microscopes available at the expertise centre.

Combined patch clamp and epifluorescence microscope for single channel studies

Instrument specifications

This microscope is designed to follow fluorescence signals such as from a calcium indicator while performing a patch clamp experiment. Imaging occurs on the single cell level. As this microscope is not a commercially available instrument, contact us for more information.

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent

Resonant scanning confocal microscope (Nikon Eclipse TE300)

Instrument specifications

Microscope designed for Fluorescence recovery after photobleaching (FRAP) experiments with two excitation lasers. A description of this microscope as it was build originally can be found in the following article.

  • Leybaert, L. , De Meyer, A. , Mabilde, C. and Sanderson, M. J. (2005), A simple and practical method to acquire geometrically correct images with resonant scanning‐based line scanning in a custom‐built video‐rate laser scanning microscope. Journal of Microscopy, 219: 133-140. doi:10.1111/j.1365-2818.2005.01502.x
  • Contact us for more information.

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent

Epifluorescence microscope for uncaging (Nikon Eclipse TE2000-E)

Instrument specifications

Microscope designed for uncaging by UV excitation. More information about uncaging and this microscope can be found in following articles or contact us.

  • Decrock E., De Bock M., Wang N., Bol M., Gadicherla A.K., Leybaert L. (2015), Flash photolysis of caged IP3 to trigger intercellular Ca2+ waves. Cold Spring Harb Protoc, 3:289-92. doi: 10.1101/pdb.prot076570.
  • Leybaert L., Sanderson MJ. (2001), Intercellular calcium signaling and flash photolysis of caged compounds. A sensitive method to evaluate gap junctional coupling. Methods Mol Biol. 154:407-30.

Location

Faculty of Medicine and Health Sciences,

Department of Basic Medical Sciences,

C. Heymanslaan 10, Blok B,

B-9000, Ghent