Affiliated Partners

Zeiss AxioZoom.V16: Fluorescence Stereo Zoom Microscope for Large Fields

Instrumentation

• Microscope
  •  Axio Zoom.V16 microscope body
  • apochromatic motor zoom 16:1 (0.7x … 11.2x)
  • Eyepiece PL 10x/23 Br foc
• 2 Objective's:
  • PlanApo Z 0.5x/0.125
  • Apo Z 1.5x FWD 30mm
• Brightfield and fluorescence illumination
• Filter set:
  • DAPI, GFP, mRFP 
• Camera Axiocam 305 color
• Zeiss image acquisition and processing software

Location

• Faculty of Veterinary Medicine, Department of Morphology, Salisburylaan 133 D5 entrance 78, B-9820 Merelbeke

Contact information

Prof. Ward De Spiegelaere

Fluorescent biosensor imaging

Expertise

Monitoring and quantitative analysis of signaling dynamics via existing/optimized or newly developed genetically encoded reporters and FRET-based biosensors in (single) living cells and tissues. (more information on the Laboratory webpage)

Instrumentation

Wide-field Nikon TiE inverted microscope with:

• Objectives: 10x and 20x (0.5 NA)
• Illumination: Lumencor Spectra X LED Light Engine
• Emission Filter sets and Dichroics (Chroma Technology) on automated filter wheel Lambda 10-B Smart Shutter (Sutter Instrument)
• Camera : DS-Qi2 CMOS camera (Nikon), 4908 x 3264 pixels
• OkoLab topstage incubation for CO2 and temperature control
• Software: Nikon NIS-Elements with JOBS module (Nikon)

Details Equipment configuration 

Location

VIB/UGent Center for Inflammation Research (IRC)

Room W278

Technologiepark-Zwijnaarde 71

9052 Gent-Zwijnaarde

Contact information

Prof. Dr. F. Riquet: franck.riquet@irc.vib-ugent.be