Affiliated PartnersAn overview of instrumentation and expertise available via affiliated partners is shown below:https://www.ugent.be/glim/en/networkhttps://www.ugent.be/++resource++plone-logo.svg
Affiliated Partners
An overview of instrumentation and expertise available via affiliated partners is shown below:
ONI NanoImager
Expertise
The ONI platform delivers high‑resolution nanoscale imaging with specialized expertise in extracellular vesicles (EVs) and diverse nanoparticles. Its capabilities include single‑particle tracking as well as dSTORM super‑resolution imaging, enabling precise visualization, quantification, and phenotyping at the molecular level. Combined with advanced cell‑imaging workflows, ONI allows researchers to study dynamic cellular processes and nanoparticle–cell interactions with exceptional clarity.
Instrument
Objective lens: Standard 100× NA 1.45 oil immersion
Field of View: 50 μm × 80 μm.
Resolution: Achievable <20 nm (SMLM).
4 high‑power lasers (DPSS/diode):
405 nm (150–1000 mW)
488 nm (200–1000 mW)
561 nm (200–500 mW)
640 nm (300–1000 mW)
Imaging Modes:
dSTORM, PALM, PAINT
Single‑molecule imaging & localization
Single‑particle tracking (SPT)
smFRET
Epi / HILO / TIRF illumination
Live‑cell compatible with temperature control up to 42°C.
Location
CORE Extracellular Vesicles, The CORE (Entrance 37A – Second floor), Campus UZ Gent, Corneel Heymanslaan 10, 9000 Ghent, Belgium
Nanoparticle Tracking Analysis (NTA) is widely used to characterize extracellular vesicles (EVs) and other nanoscale particles by directly visualizing their Brownian motion in suspension. By tracking individual particles in real time, NTA provides size distributions, concentration measurements, and insights into sample heterogeneity that are difficult to obtain with bulk methods. Furthermore, the Zetaview Evolution Twin allows for zeta potential analysis, a measure for the electrical charge at the surface of particles in a liquid.
Instruments
NanoSight LM10
Measures
Particle size distribution
Particle concentration
Fluorescently labelled particles (488 nm)
Laser: 488 nm
Compatible with syringe pump
2. ZetaviewEvolution Twin
Measures
Particle size distribution
Particle concentration
Zetapotential
Fluorescently labelled particles (488 nm, 640 nm)
Lasers: 488/640 nm
Software‑controlled pumps for automated sample loading.
Sample temperature control from –5°C to 55°C.
Fully automated focus, laser alignment, and camera calibration
Location
CORE Extracellular Vesicles, The CORE (Entrance 37A – Second floor), Campus UZ Gent, Corneel Heymanslaan 10, 9000 Ghent, Belgium
Virtual microscope - Slide scanner @Biobank UZ Gent
Instrumentation
Introducing the PANNORAMIC 250 Flash III: Advancing Pathology with Digital Precision
The Biobank of UZ Gent proudly presents the PANNORAMIC 250 Flash III, a digital microscope revolutionizing pathology. With high-resolution imaging for both brightfield and fluorescent sections, this device offers unparalleled versatility.
Equipped with four fluorescent filters—FITC, TRITC, DAPI, and Cy5—it delves into cellular dynamics with clarity. Instant evaluation through the CaseViewer program streamlines analysis, while seamless storage on the UZ Gent server, accessible via Artemis, ensures data security and accessibility.
In short, the PANNORAMIC 250 Flash III redefines pathology with precision, efficiency, and innovation
Location
Campus UZ gent
Contact information
If you have any questions or are interested in the services provided by the Biobank of UZ Gent, please feel free to contact us at the following email address: biobank@uzgent.be.
Zeiss AxioZoom.V16: Fluorescence Stereo Zoom Microscope for Large Fields
Instrumentation
Microscope
Axio Zoom.V16 microscope body
apochromatic motor zoom 16:1 (0.7x … 11.2x)
Eyepiece PL 10x/23 Br foc
2 Objective's:
PlanApo Z 0.5x/0.125
Apo Z 1.5x FWD 30mm
Brightfield and fluorescence illumination
Filter set:
DAPI, GFP, mRFP
Camera Axiocam 305 color
Zeiss image acquisition and processing software
Location
Faculty of Veterinary Medicine, Department of Morphology, Salisburylaan 133 D5 entrance 78, B-9820 Merelbeke
Monitoring and quantitative analysis of signaling dynamics via existing/optimized or newly developed genetically encoded reporters and FRET-based biosensors in (single) living cells and tissues. (more information on the Laboratory webpage)
Instrumentation
Wide-field Nikon TiE inverted microscope with:
Objectives: 10x and 20x (0.5 NA)
Illumination: Lumencor Spectra X LED Light Engine
Emission Filter sets and Dichroics (Chroma Technology) on automated filter wheel Lambda 10-B Smart Shutter (Sutter Instrument)
Camera : DS-Qi2 CMOS camera (Nikon), 4908 x 3264 pixels
OkoLab topstage incubation for CO2 and temperature control
Software: Nikon NIS-Elements with JOBS module (Nikon)
