Welcome to Enzymares

Introduction

Enzymes are everywhere - so is opportunityEnzymares logo

Enzymes power life. They break down food in our bodies, make bread rise, clean our clothes, and enhance everything from animal feed to cosmetics. With their vast applications and sustainable profile, the global demand for new and better-performing enzymes is skyrocketing.

Nature holds the key — especially marine environments, where extreme conditions like high pressure, temperature, and salinity have shaped unique enzymes with extraordinary potential. But finding and optimizing these enzymes for industrial use is a slow, complex, and inefficient process. Most discovered enzymes never make it past screening. That’s where Enzymares comes in.

Revolutionizing Enzyme Discovery

Our enzyme prediction toolbox accelerates enzyme discovery and reduces the need for trial and error. We streamline the entire process by focusing on three key areas:

  1. Smart Data Collection – Unlocking enzyme-rich biological resources from nature, in particular the marine environment.
  2. Advanced AI Algorithms – Predicting enzyme function and performance with precision.
  3. Enzymes Tailored to Industrial Applications – Testing AI-identified enzymes in real conditions and optimizing production based on performance.

Enzymares for All

Enzymares makes enzyme discovery more accessible across a broad range of sectors, including food, feed, pharmaceuticals, cosmetics, aquaculture, chemicals and biorefineries, where enzymes can serve as biocatalysts in production processes or as active components in final products.

No matter your role — whether you're developing, producing or using enzymes — Enzymares is here to support your needs. Whether you're seeking a completely new enzyme, looking to improve an existing one, our tools and expertise are available to help.

If you're a researcher, industry expert, bio-engineer, bio-informatician or business leader curious about enzyme potential... let's connect, explore opportunities and innovate together!

Enzymares consortium

Project partners

Enzymares partners

The Enzymares project is made possible with the support of

Enzymares support

Toolbox

The Enzymares Toolbox is an open-source platform developed through a collaborative initiative. Designed to accelerate enzyme discovery and engineering, this toolbox integrates computational modelling with curated biological data to empower researchers in academia and industry.

The Enzymares toolbox is hosted by KU Leuven. Do you want to use the toolbox? Click on the button below to get started.

Use the toolbox

Do you want to learn more about the development process and potential uses of the toolbox? Check out the interactive visual below.

Use cases

Bacillus subtilis xylanaseXylanases

Enzymes have been used in the food industry for decades, for both process and product optimisation as well as for increasing the nutritional value of products. Xylanases play a major role in this, by (1) valorising biomass into high-quality products that have both functional and health-promoting properties, and (2) addition in cereal-based processes to achieve specific quality traits.

There is a continuous search for novel xylanases with specific traits: improved degradation of specific types of biomass (e.g. wood bark versus grain bran), producing specific biochemical structures and being able to work in specific (extreme) conditions (e.g. high temperature, high salt).

The Enzymares toolbox can be an aid by searching more specifically for xylanases that meet the desired characteristics, which can minimize the need for time-consuming experimental screening. In addition, newly discovered xylanases can be tested against the toolbox to gain initial insight into their functional possibilities.

Learn more about the Xylanase case that was used to test and validate the toolbox by clicking on the button below.

Use case Xylanases

Lipases

The potential for lipases was tested with a similar approach. More information on this case will follow soon.

Enzymares services

Biological resources for enzyme discovery

  • Access to (marine) bacterial and diatom cultures for enzyme screening, enriched with biological and environmental metadata (UGent-LM, UGent-PAE)
  • Identification of promising (marine) micro-organisms and their molecular resources in publicly available data (KU Leuven-CSB, KU Leuven-LBEG)

Advanced screening and bio-informatics

  • Enzymatic & proteomic screening: Cutting-edge assays, including activity-based protein profiling (UGent-LM, UGent-PAE, BBEPP)
  • Genomic & transcriptomic sequencing for dentification and annotation of novel enzymes (UGent-LM, UGent-PAE, UGent-VIB, BGRL)

AI-Powered enzyme discovery toolbox

  • Machine learning & AI-based approaches to accelerate enzyme discovery (KU Leuven-ESAT/STADIUS, KU Leuven-CSB)
  • Application of the toolbox to screen public databases, your own organisms or our in-house culture collections (KU Leuven-ESAT/STADIUS, KU Leuven-CSB)

From discovery to application

  • Custom tools for biocatalytic process evaluation & benchmarking (KU Leuven-LFCB, VITO)
  • Selection and evaluation of enzyme production systems, protein engineering (UGent-LM)
  • Cultivation, upscaling, extraction & purification (BBEPP)
  • Characterization of enzymes under industrial conditions (KU Leuven-LFCB, VITO)

Contact & partner expertise

General project contact: Eveline.Diopere@ugent.be

Ghent University (UGent)

Blue Growth Research Lab (BGRL) – contact: Jana.Asselman@ugent.be (website)

  • Marine biodiscovery: Molecules from the sea(-air) for human health and other applications
  • Discovery of marine enzymes for sustainable antifouling solutions
  • Key analytical and molecular expertise

Laboratory of Microbiology (LM-UGent) – contact: Bart.Devreese@ugent.be - Anne.Willems@ugent.be (website)

Laboratory of Protistology and Aquatic Ecology (PAE) – contact: Koen.Sabbe@ugent.be (website)

  • Hosting the BCCM-DCG diatom collections – provision of curated and well-characterized diatom strains for (applied) research, cryopreservation and safe deposit of important strains
  • Genetic and genomic characterization of microalgal (especially diatom) strains
  • Experimental facilities and technical support for ecophysiological and omics experiments

Ghent University – VIB (UGent-VIB)

Laboratory of Computational Regulomics – contact: Klaas.Vandepoele@psb.vib-ugent.be (website)

  • Computational biology
  • Regulatory genomics
  • Comparative genomics
  • Genome analysis

KU Leuven

ESAT - STADIUS – contact: Bart.Demoor@esat.kuleuven.be (website)

  • Linear and multi-linear algebra, statistics, optimization, machine learning, artificial intelligence
  • Mathematical engineering tools and numerical algorithms

Computational Systems Biology (CSB) – contact:  csb@kuleuven.be – Vera.Vannoort@kuleuven.be (website)

  • Bioinformatics, computational biology, biological data analysis, proteins, microbial systems
  • Modelling, simulations, bioinformatics tool development, applied machine learning and artificial intelligence

Laboratory of Biodiversity and Evolutionary Genomics (LBEG) – contact: Filip.Volckaert@kuleuven.be (website)

  • DNA identification (metabarcoding) of marine prokaryote and eukaryote organisms
  • Population genomic tools for eukaryote host organisms
  • Authentication of marine eukaryote organisms

Laboratory of Food Chemistry and Biochemistry (LFCB) – contact: Kristof.Brijs@kuleuven.be – Christophe.Courtin@kuleuven.be (website)

  • Analysing raw materials, intermediate and end food products of mainly cereals
  • Characterisation of enzymes under industrial conditions from kinetic studies, product qualification and quantification to industrial applications
  • Mapping the conversion of cereal constituents during processing and their impact on the quality of the end product

Bio Base Europe Pilot Plant (BBEPP)

Contact: Karel.de.Winter@bbeu.orgEvelien.Uitterhaegen@bbeu.org (website)

  • Development of optimized fermentation and downstream purification processes for the recombinant production of industrial enzymes
  • Optimisation and intensification of various biocatalytic processes and product separation technologies
  • Development and fine-tuning of various enzyme activity assays to allow efficient screening and evaluation of enzyme performance
  • Scale-up of fermentation, DSP, and biocatalysis to assess industrial performance and allow kg- to ton-scale production for initial pilot testing 

Flemish Institute for Technological Research (VITO)

Contact: Heleen.Dewever@vito.be (website)

  • Kinetic studies for (trans)esterifications, performed in solvent-free conditions
  • New insights in biocatalytic reaction mechanisms
  • Novel approach for benchmarking new enzymes versus commercially available ones
  • Techno-economic evaluations
  • Kilogram-scale conversions with integrated removal of inhibitory products
  • Engineering support and modeling, including consultancy for the upscaling process