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Genomics

Massively parallel sequencing enables the accurate determination of genome sequences. Depending on your research goals and specific requirements, you can opt for whole-genome sequencing, or focus on targeted sequencing of specific genes or regions, such as exomes.

Transcriptomics

Transcriptomics involves studying the complete set of RNA transcripts produced by specific cells or tissues under defined conditions. This includes sequencing and quantifying various RNA types, such as messenger RNAs (mRNAs), microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). The resulting data enable comprehensive differential gene expression analysis, providing insights into cellular processes and regulatory mechanisms.

Epigenomics

Epigenetics research aims to uncover dynamic changes in a cell's transcriptional potential, driven by key mechanisms such as DNA methylation, histone modifications, chromatin accessibility, and non-coding RNA-associated gene regulation. The UGent NGS Core is fully equipped with advanced technologies and expertise to perform comprehensive, integrated analyses of these epigenetic mechanisms.

Bioinformatics

The UGent NGS Core, in partnership with the NXTGNT lab, offers expert support for sequencing data analysis through a dedicated team of highly skilled professionals. We handle the entire process, from raw sequencing reads to publication-ready results, including statistical analysis, data visualization, PCA, Gene Set Enrichment Analysis, pathway analysis, and more. Don’t hesitate to contact us with any questions or specific project needs.

Single Cell Sequencing

Single-cell sequencing enables the analysis of genomic, transcriptomic, and epigenomic information at the resolution of individual cells. The UGent NGS Core offers end-to-end single-cell sequencing solutions, including 10X technology, Illumina Fluent BioSciences technology, and smart-seq v3 express plate-based technology.

Laser-Capture Microdissection (LCM)

Our PALM MicroBeam system offers advanced laser microdissection capabilities for precise isolation of cells or tissue regions, enabling high-quality downstream DNA and RNA analysis. Whether working with live cells, cryosections, or FFPE tissue on standard glass slides, this versatile technology ensures reliable sample collection for molecular research.

Low Input Samples

The UGent NGS Core specializes in processing low-input samples, including single-cell samples, FFPE (formalin-fixed, paraffin-embedded) tissues, and laser capture microdissection (LCM) samples. Our expertise ensures high-quality sequencing results even from challenging or limited starting materials.

Olink proteomics

Olink proteomics technology is a high-throughput, multiplex immunoassay platform that uses Proximity Extension Assay (PEA) technology to measure protein biomarkers with exceptional specificity and sensitivity. By utilizing dual antibody binding and DNA-coupled detection, it enables robust protein quantification across a broad dynamic range, supporting applications in biomarker discovery and clinical research.

Long Read Sequencing

Oxford Nanopore sequencing directly reads unamplified DNA and RNA, eliminating the need for amplification steps and thereby avoiding the quantitative and qualitative biases introduced by amplification. It enables long reads of up to 100 kbp and provides direct detection of epigenetic modifications, such as 5-methylcytosines in DNA and N6-methyladenines in RNA, making it ideal for applications like de novo genome assembly, large structural variant detection, and RNA isoform analysis.

In addition, PacBio Revio technology offers highly accurate long-read sequencing (HiFi reads) with read lengths averaging 15–20 kbp and up to 99.9% accuracy. Its ability to generate phased diploid assemblies and resolve complex regions of the genome, including repeats and GC-rich areas, makes it a complementary tool for high-resolution genomic studies. Combined, these cutting-edge platforms empower researchers to tackle a wide range of genomic challenges with unparalleled precision and completeness.