Monica Fresta
CV
Monica Fresta graduated in MSc. Pharmaceutical and Technological Chemistry at University of Catania (Italy) on 09.07.2020 with first class of honours (110/110), a 5-year course (the equivalent to a 3-year degree and 2-year master) which included several chemical, medical and biological subjects.
During these years she not only improved technical skills in chemistry (analytical methods like NMR, HPLC, MS, and chemical probes) but also, enhanced her understanding of the whole drug discovery and development process, giving an insight on the needs of the pharmaceutical industry, and how to optimize times and reduce cost.
Her first research was the MSc. Thesis: Functionalised POSS based composites for Bone Tissue Engineering: synthesis, computational and preliminary biological studies.
The thesis project was focused in three aspects:
- Experimental part: Synthesis, characterization, and functionalisation through 1,3 dipolar cycloaddition of heterocyclic compounds containing an inorganic matrix;
- Computational part: DFT mechanistic studies of the cycloaddition in order to rationalize the regio- and stereoselectivity, In collaboration with University of Milan
- Biological part: Drug release studies and preliminary biological assays.
This thesis project led to her first publication: Functionalised polyhedral oligosilsesavioxane (POSS) based composites for bone tissue engineering: synthesis, computational and biological studies.
She also performed an internship for 6 months in a pharmacy during the master degree; and also obtained a pharmacist professional licences.
During the years of thesis, she participated in various conferences
- Sixth international conference on multifunctional, hybrid and nanomaterials - Elsevier, Sitges, Spain;
- xvii annual meeting of the pericyclic reaction centre
- in memory of Prof. Pierluigi Caramella, Pavia
- Delegate of University of Catania at "1st virtual symposium on pericyclic reactions and synthesis of carbo- and heterocyclic systems"
This was followed by a “research stay” for 6 months in the group of Dr. Silvia Osuna in Girona, Spain, mainly sponsored by HPC-Europe program. This period improved her knowledge in the field of computational chemistry, while working on the simulation of different Squalene Hopene Cyclase variants. This work was performed in collaboration with the experimental group of from Prof. Bernhard Hauer (University of Stuttgart, Germany). This involved studying the conformational dynamics of four variants that improve the catalytic activity towards the cyclization of our enzyme (Homofarnesol) to the Wild-type enzyme, and included the FEL (Free Energy Landscape) to analyse the different structure of variants. The next step was to analyse how the enzyme tunnels are affected by the presence of the introduced mutations on the enzyme sequence. For that purpose, we will use the Caver software, and the results will serve to rationalize the effect of the mutations in the cyclization of Homofarnesol. This research stay was then followed by Monica's first talk, in Girona, Spain: Computational studies of Squalene-Hopene Cyclase variants for the synthesis of terpenoid derivatives.Currently, Maylin is a doctoral researcher in the OBCR group and she is dedicated to addressing key challenges associated with CLiPs, particularly their limited solubility and non-selectivity for specific target cells.
Research Project
Currently, Monica is a doctoral researcher in the OBCR group and she focuses on understanding the mechanisms underlying all reactions that involve bioconjugation processes between organic molecules and 5HP2O through thiol compounds. The goal is to determine, by analyzing the various conformational transitions and energy states, the most probable reaction mechanism for the system under investigation.